主要研究方向
Research at Analytical DNA Nanotechnology Laboratory (ADNL) is at the interface of bioanalytical chemistry and DNA nanotechnology. We consider DNA both as important biomarkers for diverse biological processes and clinical diagnostics and also as a class of highly programmable engineering material. As such, we are interested in developing advanced tools for nucleic acid testing and protein detection at decentralized conditions, such as for point-of-care diagnosis and field-deployable tests. We are also interested in the fundamentals and applications of DNA nanotechnology, where we program DNA into diverse nanodevices and nanomachines with predictable behaviors and function.
主要工作业绩
2019 Young Investigators in Analytical and Bioanalytical Science,Analytical and Bioanalytical Chemistry(Springer旗下期刊)
2019 山西省百人计划短期项目,山西省委组织部
2018 入选Ontario-China Young Scientist Exchange Program,中国科技部与加拿大安大略政府共同资助
2018 Ontario Early Researcher Award,加拿大安大略省政府
2016 Emerging Investigator in Analytical Science,Analyst(英国皇家化学会旗下期刊)
2014 JDRF Postdoctoral Fellowship(declined),美国糖尿病协会
2013 Doctoral Dissertation Award(推荐人:Analytical Chemistry主编Jonathan Sweedler教授),University of Alberta
2013 国家优秀自费留学生奖,中国教育部
2013 Bill Davidson Graduate Student Travel Award, 7th International Symposium on Enabling Technologies
2012 Young Scientist Travel Award, 28th International Symposium on Microscale Separations and Bioanalysis
2012 J. Gordin Kaplan Graduate Student Award, University of Alberta
2012纳米技术创业大赛二等奖,University of Alberta
代表性成果 (获奖成果、专著、论文、专利)
完整文章列表见小组主页(*代表通讯作者; #代表共同一作)
1. Mason, S. D.; Wang, G. A.; Yang, P.; Li, Y.; Li, F.* Probing and controlling dynamic interactions at biomolecule-nanoparticle interfaces using stochastic DNA walkers. ACS Nano, 2019, 13, 8106-8113.
2. Li, Y.; Mansour, H.; Wang, T.; Poojari, S.; Li, F.* Naked-eye detection of grapevine red-blotch viral infection using a plasmonic CRISPR Cas12a Assay. Anal. Chem. 2019, 91, 11510-11513.
3. Li, Y.; Wang, G, A.; Mason, S. D.; Yang, X.; Yu, Z.; Tang, Y.; Li, F.* Simulation-guided engineering of an enzyme-powered three-dimensional DNA nanomachine for discriminating single nucleotide variants. Chem. Sci. 2018, 9, 6434-6439.
4. Li, F.;* Lin, Y.; Lau, A.; Tang, Y.; Chen, J.; Le, X. C.* Binding-induced molecular amplifier as a universal detection platform for biomolecules and biomolecular interaction. Anal. Chem. 2018, 90, 8651-8657.
5. Wang, G. A.; Dong, T.; Mansour H.; Matamoros, G.; Sanchez, A. L.; Li, F.* Paper-Based DNA Reader for Visualized Quantification of Soil-Transmitted Helminth Infections. ACS Sens. 2018, 3, 205-210.
6. Yang, X.; Tang, Y.; Traynor, S. M.; Li, F.* Regulation of DNA strand displacement using allosteric DNA toehold. J. Am. Chem. Soc. 2016, 138, 14076-14082.
7. Yang, X.; Tang, Y.; Mason, S. D.; Chen, J.; Li, F.* Enzyme-powered three dimensional DNA nanomachine for DNA walking, payload release, and biosensing. ACS Nano 2016, 10, 2324-2330.
8. Li, F.*; Tang, Y.; Traynor, S. M.; Li, X.-F.; Le, X. C.* Kinetics of proximity-induced intramolecular DNA strand displacement. Anal. Chem. 2016, 88, 8152-8157.
9. Tang, Y.; Wang, Z.; Yang, X.; Chen, J.; Liu, L.; Zhao, W.; Le, X. C.; Li, F.* Constructing real-time, wash-free, and reiterative sensors for cell surface proteins using binding-induced dynamic DNA assembly. Chem. Sci. 2015, 6, 5729-5733.
10. Tang, Y.; Lin, Y; Yang, X.; Wang, Z.; Le, X. C.; Li, F.* Universal strategy to engineer catalytic DNA hairpin assemblies for protein analysis. Anal. Chem. 2015, 87, 8063-8066.
11. Li, F.;# Zhang, H.;# Wang, Z.; Newbigging, M. A.; Reid, S. M..; Li, X.-F.; Le, X. C. Aptamers facilitate amplified detection of biomolecules. Anal. Chem. 2015, 87, 274-292.
12. Ali, M.M.; Li, F.; Zhang Z.; Zhang, K.; Kang, D.-K.; Ankrum, J.; Le, X. C.; Zhao, W. Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine. Chem. Soc. Rev. 2014, 43, 3324-3341.
13. Zhang, H.#; Li, F.#; Dever, B.; Li, X-F.; Le, X. C. DNA-mediated homogeneous binding assays for nucleic acids and proteins. Chem. Rev. 2013, 113, 2812-2841.
14. Li, F.; Zhang, H.; Wang, Z.; Li, X.; Li, X.-F.; Le, X. C. Dynamic DNA assemblies mediated by binding-induced DNA strand displacement. J. Am. Chem. Soc. 2013, 135, 2443-2446.
15. Li, F.; Lin, Y.; Le, X. C. Binding-induced formation of DNA three-way junctions and its application in real-time protein detection and DNA strand displacement. Anal. Chem. 2013, 85, 10835-10841.
16. Zhang, H.; Li, F.; Dever, B.; Wang, C.; Li, X.-F.; Le, X.C. Assembling DNA through affinity binding to achieve ultrasensitive protein detection. Angew. Chem. Int. Ed. 2013, 52, 10698-10705.
17. Li, F.; Zhang, H.; Lai C.; Li, X-F.; Le, X. C. A molecular translator that acts by binding-induced DNA strand displacement for a homogeneous protein assay. Angew. Chem. Int. Ed. 2012, 51, 9317-9320.
18. Li, F.; Dever, B.; Zhang, H.; Li, X-F.; Le, X. C. Mesoporous materials in peptidome analysis. Angew. Chem. Int. Ed. 2012, 51, 3518-3519.
19. Li, F.; Li, J.; Wang, C.; Zhang, J.; Li, X.-F.; Le, X. C. Competitive protection of aptamer-functionalized gold nanoparticles by controlling DNA assembly. Anal. Chem. 2011, 83, 6464-6467.
20. Li, F.; Zhao, Q.; Wang, C.; Lu, X.; Li, X.-F.; Le, X. C. Detection of E. coli O157: H7 using gold nanoparticle labeling and inductively coupled plasma-mass spectrometry. Anal. Chem. 2010, 82, 3399-3403.
作者: max 时间: 2021-11-28 19:15
CRISPR基因魔剪自1980年被首次发现以来,已为化学、生物、生物医学等学科带来了革命性的变化。近几年来, 由于其独特的分子识别及信号放大机制,CRISPR被广泛应用于分析与诊断工具的开发,部分CRISPR诊断工具已成功实现临床应用。
近日,我院李峰教授发表关于CRISPR分析诊断技术的论文,系统综述了CRISPR技术在分析方法开发与分子诊断中的应用。该文从CRISPR各类工具包的独特识别与酶切性质入手,深入剖析了各类CRISPR分析诊断技术的设计理念与技术路径,全面探讨了其灵敏度、特异性、检测通量、速度等分析性能,并进一步讲述了CRISPR与纳米技术、微流控技术等的深度整合及其在新冠病毒诊断等重大公共卫生事件中的临床应用。此外,文章还深入探讨了未来CRISPR在分析诊断领域所面临的挑战、解决思路及新的展望。
该研究以“Mismatch-guided deoxyribonucleic acid assembly enables ultrasensitive and multiplex detection of low-allele-fraction variants in clinical samples”为题发表在Journal of the American Chemical Society上,第一作者是四川大学化学学院博士研究生黄丹,华西呼吸健康研究所邓慧博士和华西检验科周娟博士。化学学院李峰教授、华西医院李为民教授、应斌武教授为共同通讯作者。